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unveils the Escherichia coli proteome on a microarray scale.","description":"We successfully identified the proteome expressed in Escherichia coli (E. coli) cells on a microarray scale using one-dimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS\/MS) with a 350 cm long, 100 microm i. d., monolithic silica-C(18) capillary column. E. coli tryptic digest (4 microg) was injected onto the column, and a 41 h gradient was applied with a flow rate of 500 nL\/min at less than 20 MPa. In total, 22,196 nonredundant tryptic peptides from 2602 proteins, including 830 membrane proteins, were identified from the E. coli cells (triplicate analysis), in which an equivalent number of genes was detected by transcriptome analysis. Approximately a 5-fold larger peak response on average was obtained in this system, compared with that obtained by conventional capillary LC-MS\/MS analysis with a 15 cm long, 3 microm diameter C(18) silica particle-packed column. The higher response suggests that the influence of ionization suppression was drastically reduced by the high-efficiency separation on the long monolithic silica column coupled with the shallow gradient. 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Here, we present the first protocol for quantitative phosphoproteome analysis of FFPE sections by utilizing phase-transfer surfactant-aided extraction\/tryptic digestion of FFPE proteins followed by high-recovery phosphopeptide enrichment via lactic acid-modified titania chromatography. We established that FFPE sections retain a similar phosphoproteome to fresh tissue specimens during storage for at least 9 months, confirming the utility of our method for evaluating phosphorylation profiles in various diseases. We also verified that chemical labeling based on reductive dimethylation of amino groups was feasible for quantitative phosphoproteome analysis of FFPE samples on slides. Furthermore, we improved the LC\u00e2\u20ac\u201cMS sensitivity by miniaturizing nanoLC columns to 25 \u00ce\u00bcm inner diameter. With this system, we could identify 1090 phosphopeptides from a single FFPE section obtained from a microscope slide, containing 25.2 \u00c2\u00b1 5.4 \u00ce\u00bcg of proteins. This protocol should be useful for large-scale phosphoproteome analysis of archival FFPE slides, especially scarce samples from patients with rare diseases.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Yasushi Ishihama"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto University"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Hironori Achiwa"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto Univ."}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"FFPE slide; formalin-fixed and paraffin-embedded; phase-transfer surfactant; phosphoproteome analysis; post-translational modifications; protein extraction; shotgun analysis"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Mus musculus 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Molecular mechanisms for the adipose-selective activation of thermogenesis remain poorly understood. Here, we employed quantitative phosphoproteomics to map global and temporal phosphorylation profiles in brown, beige, and white adipocytes under \u00ce\u00b23-adrenenoceptor activation and identified kinases responsible for the adipose-selective phosphorylation profiles. We found that casein kinase2 (CK2) activity is preferentially higher in white adipocytes than brown\/beige adipocytes. Genetic or pharmacological blockade of CK2 in white adipocytes activates the thermogenic program in response to cAMP stimuli. Such activation is largely through reduced CK2-mediated phosphorylation of class I HDACs. Notably, inhibition of CK2 promotes beige adipocyte biogenesis and leads to an increase in whole-body energy expenditure and ameliorates diet-induced obesity and insulin resistance. These results indicate that CK2 is a plausible target to rewire the \u00ce\u00b23-adrenenoceptor signaling cascade that promotes thermogenesis in adipocytes.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Shingo Kajimura"},{"accession":"MS:1000590","name":"contact affiliation","value":"University of California, San Francisco"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Natsumi Ishikawa"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyot Univ."}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"Beige adipocyte"},{"accession":"MS:1001925","name":"submitter keyword","value":"phosphoproteomics"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Mus musculus (Mouse)"},{"accession":"MS:1001467","name":"taxonomy: NCBI TaxID","value":"10090"}]}],"instruments":[],"modifications":[],"fullDatasetLinks":[{"accession":"MS:1002852","name":"Dataset FTP location","value":"ftp:\/\/ftp.jpostdb.org\/JPST000096"},{"accession":"MS:1002633","name":"jPOST dataset URI","value":"https:\/\/repository.jpostdb.org\/entry\/JPST000096"}],"datasetFiles":[{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000096\/150528aki_bg0.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000096\/150528aki_bg5.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000096\/150528aki_bg10.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000096\/150528aki_bg20.raw"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000096\/quant.txt"}]},{"accession":[{"name":"jPOST dataset identifier","value":"JPST000097","accession":"MS:1002632","cvLabel":"MS"},{"name":"ProteomeXchange accession number","value":"","accession":"MS:1001919","cvLabel":"MS"}],"title":"Brown adipocyte phosphoproteomics","description":"Catecholamines promote lipolysis both in brown and white adipocytes, whereas the same stimuli preferentially activate thermogenesis in brown adipocytes. 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Mouse thymocytes were exposed to four different conditions\u00e2\u20ac\u201ddimethyl sulfoxide (DMSO, vehicle control), CsA alone, IOM alone, and both CsA and IOM\u00e2\u20ac\u201dand were then compared with regard to global phosphorylation status. Phorphoproteomics analysis identified and quantified  unique phosphopeptides corresponding tophosphorylation sites of 2634 proteins.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Masaki Matsumoto"},{"accession":"MS:1000590","name":"contact affiliation","value":"Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University,"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Masaki Matsumoto"},{"accession":"MS:1000590","name":"contact affiliation","value":"Dep. Omics and Sys. 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This version of the database was created from the results of 27 nanoLC-MS\/MS runs on a hybrid ion trap-orbitrap mass spectrometer, which offers high accuracy for analyzing tryptic digests from undifferentiated cultured rice cells. Peptides were identified by searching the product ion spectra against the protein, cDNA, transcript and genome databases from Michigan State University, and were mapped to the rice genome. Approximately 3200 genes were covered by these peptides and 40 of them contained novel genomic features. Users can search, download or navigate the database per chromosome, gene, protein, cDNA or transcript and download the updated annotations in standard GFF3 format, with visualization in PNG format. In addition, the database scheme of OryzaPG was designed to be generic and can be reused to host similar proteogenomic information for other species. OryzaPG is the first proteogenomics-based database of the rice proteome, providing peptide-based expression profiles, together with the corresponding genomic origin, including the annotation of novelty for each peptide.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Yasushi Ishihama"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto University"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Natsumi Ishikawa"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyot Univ."}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"Rice"},{"accession":"MS:1001925","name":"submitter keyword","value":"Oryza sativa"},{"accession":"MS:1001925","name":"submitter keyword","value":"proteogenomics"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific 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identifier","value":"JPST000159","accession":"MS:1002632","cvLabel":"MS"},{"name":"ProteomeXchange accession number","value":"PXD004698","accession":"MS:1001919","cvLabel":"MS"}],"title":"Zinc-responsive labeling in live cells for novel conditional proteomics","description":"Zinc signaling and dynamics play significant roles in many physiological responses and diseases. To understand the physiological roles of zinc in detail, comprehensive identification of proteins under high concentration of mobile zinc ion is crucial. In this report, we developed a novel \u00e2\u20ac\u0153conditional proteomics\u00e2\u20ac\u009d based on a chemical proteomic strategy with designer zinc-responsive labeling reagents (AIZin). In this conditional proteomics, a subset of proteins in a zinc-rich environment is tagged and identified in combination with quantitative mass spectrometry. This method was successfully applied to elucidate zinc dyshomeostasis induced by nitric oxide-triggered oxidative stress in glioma cells and unveiled dynamic changes of the zinc-related proteomes. Moreover, a subset of protein composition identified by our method allowed the characterization of unknown zinc-rich vesicles generated by oxidative stress as endoplasmic reticulum-Golgi-related vesicles.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Itaru Hamachi"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto University"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Hironori Achiwa"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto Univ."}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"conditional proteomics"},{"accession":"MS:1001925","name":"submitter keyword","value":"chemical proteomics"},{"accession":"MS:1001925","name":"submitter keyword","value":"TMT"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Rattus rattus (Black rat)"},{"accession":"MS:1001467","name":"taxonomy: NCBI TaxID","value":"10117"}]}],"instruments":[],"modifications":[],"fullDatasetLinks":[{"accession":"MS:1002852","name":"Dataset FTP location","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159"},{"accession":"MS:1002633","name":"jPOST dataset URI","value":"https:\/\/repository.jpostdb.org\/entry\/JPST000159"}],"datasetFiles":[{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/150710mw14_TMT1.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/150710mw15_TMT2.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/150906mw02_TMT010180_1.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/150906mw03_TMT010180_2.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/151001mw02_TMT010180_1.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/151001mw03_TMT010180_2.raw"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/150710mw14_TMT1.mgf"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/150710mw15_TMT2.mgf"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/150906mw02_TMT010180_1.mgf"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/150906mw03_TMT010180_2.mgf"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/151001mw02_TMT010180_1.mgf"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/151001mw03_TMT010180_2.mgf"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/F014517.mzid"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/F014518.mzid"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/F014519.mzid"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/F014520.mzid"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/F014523.mzid"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000159\/F014524.mzid"}]},{"accession":[{"name":"jPOST dataset identifier","value":"JPST000160","accession":"MS:1002632","cvLabel":"MS"},{"name":"ProteomeXchange accession number","value":"PXD004633","accession":"MS:1001919","cvLabel":"MS"}],"title":"Proteomic Comparison of the Secreted Factors of Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue and Dental Pulp","description":"Mesenchymal stem cells (MSCs) have therapeutic potential in various diseases, including myocardial infarction. Recently, the importance of the therapeutic effects of secreted factors from MSCs is increasing, but their identification has not progressed. In this study, we uncover the secreted protein profiles of MSCs derived from bone marrow, adipose tissue and dental pulp. Shotgun proteome analysis of conditioned MSC media by mass spectrometry identified 1533 proteins totally and 124 secreted proteins commonly produced among all three MSCs. The commonly secreted factors include already well-known factors whose functions are linked to MSCs\u00e2\u20ac\u2122 biological effects, such as CTGF, SERPINE1, TGFB1, DKK3 and MYDGF, and also include newly identified factors whose roles are not well investigated, for example AIMP1, CLEC11A, GAS6, HDGF, INHBA, and PCSK5. Computational biological pathway analysis revealed that these common factors strongly relate to tissue regeneration pathways such as angiogenesis, migration, and inflammatory response. Further analysis showed enrichment of ossification, sprouting, and organ survival factors, suggesting connection to the functions closely related to MSCs\u00e2\u20ac\u2122 therapeutic effects. This list of commonly secreted proteins could provide a reliable resource of biological factors which explain various effects of MSCs and would be useful for identifying new therapeutic factors produced from MSCs.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Kazuishi Kubota"},{"accession":"MS:1000590","name":"contact affiliation","value":"Daiichi Sankyo RD Novare Co., Ltd."}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Hidetaka Sakurai"},{"accession":"MS:1000590","name":"contact affiliation","value":"Daiichi Sankyo RD Novare co., ltd"}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"BMSC"},{"accession":"MS:1001925","name":"submitter keyword","value":"DPSC"},{"accession":"MS:1001925","name":"submitter keyword","value":"ASC"},{"accession":"MS:1001925","name":"submitter 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Along those lines, optimization of proteomics workflow toward  limited number of cells is an asset. Here, we report our workflow of microproteomics, proteomics for a limited number of cells: a microfluidic-chip based cell sorter was applied for a damage-free cell sorting, and each 100 or 1000 THP-1 cells were collected in hydrophilic or albumin-coated microtubes. Following in-solution enzymatic digestions, peptide mixtures were purified and applied to nanoLC-MS (Orbitrap  Elite) analysis with 3 hr gradient. Our method enabled proteome mapping from 1000-sorted cells, with a similar in-depth map from 1 \u00ce\u00bcg of cell lysate. This approach would be useful for cell subsets and limited materials such as circulating tumor cells, inflammatory cells in cancer tissues and clinical materials.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Kie Kasuga"},{"accession":"MS:1000590","name":"contact affiliation","value":"Niigata University of Pharmacy and Applied Life Sciences"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Kie Kasuga"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kanazawa University"}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"THP-1"},{"accession":"MS:1001925","name":"submitter keyword","value":"shotgun proteomics"},{"accession":"MS:1001925","name":"submitter keyword","value":"Cell-sorting"},{"accession":"MS:1001925","name":"submitter 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metabolism-related enzymes were obtained by the selected reaction monitoring (SRM) assay methods using LC-MS\/MS from the wild type (BY4742), a GCR2 gene deletion strain, and 29 single-gene deletion strains lacking enzyme genes responsible for central carbon metabolism (including CIT1, ENO1, FBP1, GCR2, GND1, GPD1, GPM2, HOR2, HXK1, HXK2, IDH1, IDH2, IDP1, LPD1, MAE1, MDH1, MDH2, PDA1, PDC1, PFK1, PYC2, RPE1, TAL1, TDH1, TDH2, TDH3, TKL1, TPS1, TPS2, and ZWF1 genes).&#10;The central carbon metabolism is strictly controlled by modulation of enzyme expressions to maintain an essential system of living organisms. In this study, metabolic safety mechanisms in the model organism, Saccharomyces cerevisiae, were investigated by direct determination of enzyme expression levels. Targeted proteome analysis of 31 S. cerevisiae wild type and mutant strains revealed that at least 30% of the observed variations in enzyme expression levels could be explained by global regulatory mechanisms. Co-expression analysis revealed that expression levels of enzymes involved in trehalose metabolism and glycolysis changed in a coordinated manner under the control of the transcription factors for global regulation. 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Both were separated by high-resolution 2-DE with a gel size of 23 cm x 30 cm and stained with FireSilver. 3 spots of the spermatophylax were excised, trypsin digested and analyzed by LC-FTICR.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Peter Jungblut"},{"accession":"MS:1000590","name":"contact affiliation","value":"MPIIB"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Peter Jungblut"},{"accession":"MS:1000590","name":"contact affiliation","value":"MPIIB"}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"nuptial gift"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Poecilimon ampliatus"},{"accession":"MS:1001467","name":"taxonomy: NCBI TaxID","value":"473668"}]}],"instruments":[],"modifications":[],"fullDatasetLinks":[{"accession":"MS:1002852","name":"Dataset FTP location","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190"},{"accession":"MS:1002633","name":"jPOST dataset URI","value":"https:\/\/repository.jpostdb.org\/entry\/JPST000190"}],"datasetFiles":[{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/Phylax8CBB_17.mzXML"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/Phylax8CBB_18.mzXML"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/Phylax8CBB_19.mzXML"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/MS2_Phylax8-17.mgf"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/MS2_Phylax8-18.mgf"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/MS2_Phylax8-19.mgf"},{"accession":"PRIDE:0000410","name":"Other type file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/2DE Gel_analysierte SpotsPF.ppt"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/MS2_Phylax8-17.xml"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/MS2_Phylax8-18.xml"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000190\/MS2_Phylax8-19.xml"}]},{"accession":[{"name":"jPOST dataset identifier","value":"JPST000191","accession":"MS:1002632","cvLabel":"MS"},{"name":"ProteomeXchange accession number","value":"PXD005027","accession":"MS:1001919","cvLabel":"MS"}],"title":"Spermatophylax of Poecilimon ampliatus liquid sample","description":"The spermatophore was removed and separated into ampulla and spermatophylax. Each of these compartments was solubilized in 6M urea, 3 M thiourea, 70 mM DTT and centrifuged. The supernatant was analyzed directly by LC-FTICR MS.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Peter Jungblut"},{"accession":"MS:1000590","name":"contact affiliation","value":"MPIIB"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Peter Jungblut"},{"accession":"MS:1000590","name":"contact affiliation","value":"MPIIB"}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"bush cricket spermatophore"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Poecilimon ampliatus"},{"accession":"MS:1001467","name":"taxonomy: NCBI TaxID","value":"473668"}]}],"instruments":[],"modifications":[],"fullDatasetLinks":[{"accession":"MS:1002852","name":"Dataset FTP location","value":"ftp:\/\/ftp.jpostdb.org\/JPST000191"},{"accession":"MS:1002633","name":"jPOST dataset URI","value":"https:\/\/repository.jpostdb.org\/entry\/JPST000191"}],"datasetFiles":[{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000191\/140614_Lehmann_Phylanx.mzXML"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000191\/LTQ_140614_Lehmann_Phylanx_liquid.mgf"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000191\/Phylax_liquid.xml"}]},{"accession":[{"name":"jPOST dataset identifier","value":"JPST000193","accession":"MS:1002632","cvLabel":"MS"},{"name":"ProteomeXchange accession number","value":"PXD005029","accession":"MS:1001919","cvLabel":"MS"}],"title":"Ampulla of Poecilimon ampliatus liquid sample","description":"The spermatophore was removed and separated into ampulla and spermatophylax. Each of these compartments was solubilized in 6M urea, 3 M thiourea, 70 mM DTT and centrifuged. 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Each of these compartments was solubilized in 6M urea, 3 M thiourea, 70 mM DTT and centrifuged. The supernatant was separated by 2-DE and spots 9,10, and 11 were analyzed by LC-FTICR MS. Spots 9,10, and 11 were occuring on 2-DE patterns of both spermatophylax and ampulla.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Peter Jungblut"},{"accession":"MS:1000590","name":"contact affiliation","value":"MPIIB"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Peter Jungblut"},{"accession":"MS:1000590","name":"contact affiliation","value":"MPIIB"}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"bushcricket"},{"accession":"MS:1001925","name":"submitter keyword","value":"spermatophore"},{"accession":"MS:1001925","name":"submitter keyword","value":"2-DE\/MS"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Poecilimon ampliatus"},{"accession":"MS:1001467","name":"taxonomy: NCBI TaxID","value":"473668"}]}],"instruments":[],"modifications":[],"fullDatasetLinks":[{"accession":"MS:1002852","name":"Dataset FTP location","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194"},{"accession":"MS:1002633","name":"jPOST dataset URI","value":"https:\/\/repository.jpostdb.org\/entry\/JPST000194"}],"datasetFiles":[{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194\/140613_Lehmann_ID_Spot09a.mzXML"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194\/140613_Lehmann_ID_Spot10a.mzXML"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194\/140613_Lehmann_ID_Spot11a.mzXML"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194\/FT_140613_Lehmann_ID_Spot9a.xml"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194\/FT_140613_Lehmann_ID_Spot10a.xml"},{"accession":"PRIDE:0000408","name":"Search engine output file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194\/FT_140613_Lehmann_ID_Spot11a.xml"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194\/LTQ_140613_Lehmann_ID_Spot09a.mgf"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194\/LTQ_140613_Lehmann_ID_Spot10a.mgf"},{"accession":"PRIDE:0000409","name":"Peak list file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000194\/LTQ_140613_Lehmann_ID_Spot11a.mgf"}]},{"accession":[{"name":"jPOST dataset identifier","value":"JPST000196","accession":"MS:1002632","cvLabel":"MS"},{"name":"ProteomeXchange accession number","value":"PXD005079","accession":"MS:1001919","cvLabel":"MS"}],"title":"Estimate phosphopeptide identification rate by tandem mass spectrometry","description":"Mass spectrometry has played a significant role in the identification of unknown phosphoproteins and sites of phosphorylation in biological samples. Analysis of protein phosphorylation, in particular large scale phosphorylation analysis \u00e2\u20ac\u201c phosphoproteomics, has recently been enhanced by efficient enrichment, fast and accurate instrument, and better software, but challenges remain because of low stoichiometry of phosphorylation and poor efficiency in ionization and fragmentation due to neutral loss. To evaluate current mass spectrometric performance, we present here a method to estimate phosphopeptide identification efficiency by tandem mass spectrometry. Phosphopeptides were directly isolated from whole plant cell extracts, dephosphorylated, and then incubated with three purified kinases, CK2, MAPK6, and SnRK2.6, along with 16O4- and 18O4-ATP separately, for in vitro kinase reactions. Phosphopeptides were further enriched and analyzed by LC-MS. Phosphopeptide identification rate was estimated by comparing phosphopeptides identified by tandem mass spectrometry with phosphopeptide pairs generated by stable isotope labeled kinase reactions. Overall we found that the current high speed and high accurate mass spectrometry can only identify 20-40% of total phosphopeptides mainly due to relatively poor fragmentation and low abundance.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Dr. W. Andy Tao"},{"accession":"MS:1000590","name":"contact affiliation","value":"Purdue University"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Chuan-Chih Hsu"},{"accession":"MS:1000590","name":"contact affiliation","value":"Purdue University"}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"Phosphopeptide identification rate"},{"accession":"MS:1001925","name":"submitter keyword","value":"Mass spectrometry"},{"accession":"MS:1001925","name":"submitter keyword","value":"Kinase reaction"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Arabidopsis thaliana (Mouse-ear cress)"},{"accession":"MS:1001467","name":"taxonomy: NCBI TaxID","value":"3702"}]}],"instruments":[],"modifications":[],"fullDatasetLinks":[{"accession":"MS:1002852","name":"Dataset FTP 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In some cases, older proteins are prone to be damaged and decline in their functions compared to newly synthesized proteins, likely to contribute the cellular aging. The purpose of this analysis is to uncover asymmetrically inherited older proteins, which are preferentially retained in mother cells during division into mother and rejuvenating daughter cells. To achieve this purpose, we conducted an originally developed strategy where mother and daughter cells are separated after just one cycle of synchronized culture, during which newly synthesized proteins are labeled with stable isotope amino acid. Synchronized culture was carried out using mating pheromone, which arrest the budding yeast in G1 phase. We successfully identified more than 20 proteins whose older forms are asymmetrically inherited by mother cells, among which are proteins involved in the intracellular homeostasis of the proton concentration and the response to the stress of misfolded proteins.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Keiji Kito"},{"accession":"MS:1000590","name":"contact affiliation","value":"Meiji University"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Keiji Kito"},{"accession":"MS:1000590","name":"contact affiliation","value":"Meiji University"}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"Asymmetric inheritance"},{"accession":"MS:1001925","name":"submitter keyword","value":"Protein aging"},{"accession":"MS:1001925","name":"submitter keyword","value":"Replicative 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In some cases, older proteins are prone to be damaged and decline in their functions compared to newly synthesized proteins, likely to contribute the cellular aging. The purpose of this analysis is to uncover asymmetrically inherited older proteins, which are preferentially retained in mother cells during division into mother and rejuvenating daughter cells. To achieve this purpose, we conducted an originally developed strategy where mother and daughter cells are separated after just one cycle of synchronized culture, during which newly synthesized proteins are labeled with stable isotope amino acid. Synchronized culture was carried out using virgin cells, which have never produce daughter cells and enriched in G1 phase. We successfully identified more than 20 proteins whose older forms are asymmetrically inherited by mother cells, among which are proteins involved in the intracellular homeostasis of the proton concentration and the response to the stress of misfolded proteins.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Keiji Kito"},{"accession":"MS:1000590","name":"contact affiliation","value":"Meiji University"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Keiji Kito"},{"accession":"MS:1000590","name":"contact affiliation","value":"Meiji University"}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"Asymmetric inheritance"},{"accession":"MS:1001925","name":"submitter keyword","value":"Protein aging"},{"accession":"MS:1001925","name":"submitter keyword","value":"Replicative 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cancer tissues ","description":"Quantitative phosphoproteome analyses of colorectal cancer tissues from 24 patients (12 with or without metastasis) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS\/MS with iTRAQ labeling(metastasis:iTRAQ115 or 117, no metastasis: iTRAQ114 or 116). The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR thresholds by searching against the randomized decoy DB at peptide level. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Takeshi Tomonaga"},{"accession":"MS:1000590","name":"contact affiliation","value":"National Institute of Biomedical Innovation, Japan"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Mariko Ito"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto Univ."}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"colorectal cancer; FASP; IMAC; missing protein; phosphopeptide; PhosphoSitePlus"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Homo sapiens (Human)"},{"accession":"MS:1001467","name":"taxonomy: NCBI TaxID","value":"9606"}]}],"instruments":[],"modifications":[],"fullDatasetLinks":[{"accession":"MS:1002852","name":"Dataset FTP location","value":"ftp:\/\/ftp.jpostdb.org\/JPST000204"},{"accession":"MS:1002633","name":"jPOST dataset URI","value":"https:\/\/repository.jpostdb.org\/entry\/JPST000204"}],"datasetFiles":[{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000204\/CRC_phospho_iTRAQ_01.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000204\/CRC_phospho_iTRAQ_02.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000204\/CRC_phospho_iTRAQ_03.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000204\/CRC_phospho_iTRAQ_04.raw"},{"accession":"PRIDE:0000404","name":"Associated raw file 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The obtained results were searched against UniProt DB by Mascot, and the identified peptides and proteins were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Takeshi Tomonaga"},{"accession":"MS:1000590","name":"contact affiliation","value":"National Institute of Biomedical Innovation, Japan"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Mariko Ito"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto Univ."}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"colorectal cancer; FASP; missing protein;"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Homo sapiens (Human)"},{"accession":"MS:1001467","name":"taxonomy: NCBI 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accession number","value":"PXD005174","accession":"MS:1001919","cvLabel":"MS"}],"title":"Phosphoproteomic data of HCT116 cells","description":"Quantitative phosphoproteome analyses of colorectal cancer cell lines (HCT116 metastatic clone and the parent clone without metastasis) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS\/MS with iTRAQ labeling(metastasis:iTRAQ116 or 117, no metastasis: iTRAQ114 or 115). The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR thresholds by searching against the randomized decoy DB at peptide level. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Takeshi Tomonaga"},{"accession":"MS:1000590","name":"contact affiliation","value":"National Institute of Biomedical Innovation, Japan"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Mariko Ito"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto Univ."}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"colorectal cancer; FASP; IMAC; missing protein; phosphopeptide; PhosphoSitePlus"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Homo sapiens (Human)"},{"accession":"MS:1001467","name":"taxonomy: NCBI 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The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR thresholds by searching against the randomized decoy DB at peptide level. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Takeshi Tomonaga"},{"accession":"MS:1000590","name":"contact affiliation","value":"National Institute of Biomedical Innovation, Japan"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Mariko Ito"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto Univ."}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"colorectal cancer; FASP; IMAC; missing protein; phosphopeptide; PhosphoSitePlus"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Homo sapiens (Human)"},{"accession":"MS:1001467","name":"taxonomy: NCBI 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The obtained results were searched against UniProt DB by Mascot, and the identified peptides and proteins were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.","publications":[],"contacts":[{"terms":[{"accession":"MS:1002332","name":"lab head"},{"accession":"MS:1000586","name":"contact name","value":"Takeshi Tomonaga"},{"accession":"MS:1000590","name":"contact affiliation","value":"National Institute of Biomedical Innovation, Japan"}]},{"terms":[{"accession":"MS:1002037","name":"dataset submitter"},{"accession":"MS:1000586","name":"contact name","value":"Mariko Ito"},{"accession":"MS:1000590","name":"contact affiliation","value":"Kyoto Univ."}]}],"keywords":[{"accession":"MS:1001925","name":"submitter keyword","value":"colorectal cancer; FASP; missing protein;"}],"species":[{"terms":[{"accession":"MS:1001469","name":"taxonomy: scientific name","value":"Homo sapiens (Human)"},{"accession":"MS:1001467","name":"taxonomy: NCBI TaxID","value":"9606"}]}],"instruments":[],"modifications":[],"fullDatasetLinks":[{"accession":"MS:1002852","name":"Dataset FTP location","value":"ftp:\/\/ftp.jpostdb.org\/JPST000208"},{"accession":"MS:1002633","name":"jPOST dataset URI","value":"https:\/\/repository.jpostdb.org\/entry\/JPST000208"}],"datasetFiles":[{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000208\/111222_HL02.RAW"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000208\/111222_HL03.RAW"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000208\/111222_HL04.RAW"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000208\/111222_HL05.RAW"},{"accession":"PRIDE:0000404","name":"Associated raw file URI","value":"ftp:\/\/ftp.jpostdb.org\/JPST000208\/111222_HL06.RAW"},{"accession":"PRIDE:0000404","name":"Associated raw 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colon tissues (tumor and non-tumor)","description":"Phosphoproteome analyses of colorectal cancer tissues from 20 patients (20 tumors and 20 tumor-adjacent normal tissues) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS\/MS. The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR thresholds by searching against the randomized decoy DB at peptide level. 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